Rapid field-based sugarcane tissue measurement using an integrated smartphone and lab-on-a-chip system
This project revolutionises nutrient management in the Australian sugar industry by advancing a ‘lab-on-a-chip’ (LOC) smartphone technology for real-time plant tissue nutrient analysis. Designed for affordability and ease of use, the LOC device delivers instant estimates of nitrate and phosphate concentrations in sugarcane tissues, aligning nutrient management practices with critical plant growth stages.
The LOC system features a compact plastic device containing chemical reagents tailored to specific nutrients. With built-in calibration standards for quality assurance, the technology provides accurate, on-the-spot data, offering an efficient and cost-effective alternative to traditional laboratory analyses. Its integration with smartphone technology enables seamless data visualisation and cloud-based tracking, empowering farmers and agronomists with actionable insights to optimise crop productivity while minimising environmental impact.
Soil nutrient levels can vary significantly within a field due to texture, organic matter, moisture, and microbial activity. Measuring plant tissues bypasses these complexities and reflects the cumulative effect of all soil factors influencing plant availability and nutrient uptake. By targeting sugarcane tissues such as fully expanded leaves, stalks, and juice samples during key growth stages, this portable and user-friendly LOC device accelerates nutrient testing, reduces costs, and enhances fertiliser efficiency, setting a new standard for precision agriculture in the sugarcane industry.
Acknowledgement
This project is fully funded by the Department of Primary Industries and managed by Sugar Research Australia.

Objectives
This project aims to improve nutrient management in sugarcane farming by addressing the inefficiencies of current nutrient analysis methods. Specifically, it focuses on developing a rapid, affordable, and user-friendly LOC device integrated with a smartphone application to measure key nutrients like nitrate and dissolvable phosphate directly in sugarcane tissues at various growth stages, including establishment, tillering, grand growth (vegetative stage) and maturity.
Targeting tissues such as leaves, stalks, and juice during critical phases—establishment, tillering, vegetative growth and maturity—the system will provide real-time insights to optimise fertilisation strategies. By eliminating reliance on distant laboratories, the technology simplifies the testing process, delivers instant results, and enables growers to make timely, data-driven decisions.
Key developments include a user-friendly sampling device, an enhanced smartphone app for seamless data interpretation, and customisation with input from industry partners. This innovation aims to improve yields, reduce fertiliser waste, and minimise environmental impacts, particularly nutrient runoff into sensitive ecosystems like the Great Barrier Reef. The project supports precision nutrient management, empowering growers with practical tools for sustainable and productive sugarcane cultivation.

Expected Outputs
• Enhanced LOC Device Prototype: Development of a refined prototype using advanced 3D printing for accurate soil and plant tissue analysis.
• Smartphone Application: Creation of an app for real-time colourimetric data analysis with the LOC device.
• Standardised Sampling Protocols: Establishment of protocols for different plant tissue (i.e. +3 or +4 leaves, stalks and stalk juice) processing compatible with the LOC device.
• Commercial-Grade LOC Device: Finalisation of the design for mass production using mould injection techniques.
• Published Research: Submission of research findings to scientific journals.
• Conferences: Presentations at relevant scientific and industry conferences to build awareness and support.
• Demonstration and Training Sessions: Industry workshops and field demonstrations held and feedback documented.
Expected Outcomes
Short Term
• Increased grower awareness and engagement with LOC technology.
• Successful field validation of LOC device and app.
• Establishment of industry partnerships for commercialisation.
Medium Term
• Widespread adoption of LOC technology in sugarcane farming regions.
• Improved nutrient use efficiency, leading to cost savings and better crop productivity.
• Reduction in fertiliser overuse and associated environmental impacts.
CHIEF INVESTIGATOR: Dr Liang Wang
END DATE: 31/03/2027

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